TSLP Protein (C-His, Human)

1.Recombinant Human CRLF2/TSLPR Protein, hFc Tag (Cat. No. 29749-H02H) captured on Protein A chip can bind Recombinant Human TSLP Protein (R127A, R130S), His Tag (Cat. No. 16135-H08H) with an affinity constant of 1.43 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 2.Immobilized Recombinant Human CRLF2 Protein (ECD, Fc Tag) (Cat: 29749-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human TSLP Protein (R127A, R130S, His Tag) (Cat: 16135-H08H), the EC50 is 0.8-3 ng/mL (QC tested) . 3.Immobilized Recombinant Human TSLP Protein (R127A, R130S, His Tag) (Cat: 16135-H08H) at 1 μg/mL (100 μL/well) can bind Anti-TSLP (Research Grade Tezepel*mab Biosimilar), the EC50 is 13.7 ng/mL (Routinely tested) .

The recombinant human TSLP consists of 142 amino acids and predicts a molecular mass of 16.2 kDa. As a result of glycosylation, it migrates as an approximately 24.9 KDa band in SDS-PAGE under reducing conditions.The molecular weight of this protein is around 21.4kDa verified by SEC-MALS (Routinely tested) .