TNF Protein (Native, Human)

1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. 2.Captured Anti-TNFa (Research Grade Adalim*mab Biosimilar) on Anti-human IgG Fc via CM5 Chip can TNF-α (Cat.No.10602-HNAE) with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 3.Anti-TNFa (Research Grade Adalim*mab Biosimilar) captured on Protein A chip can bind Recombinant Human TNF-alpha Protein (Cat. No. 10602-HNAE) with an affinity constant of 0.12 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 4.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested) . 5.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested) . 6.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Inflix*mab Biosimilar), the EC50 is 1.5-5.0 ng/mL (Routinely tested) . 7.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 1 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Golim*mab Biosimilar), the EC50 is 2.0-6.0 ng/mL (Routinely tested) . 8.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF1A Protein (Fc Tag) (Cat: 10872-H02H), the EC50 is 5.6-17.0 ng/mL (QC tested) . 9.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFR2 / CD120b / TNFRSF1B Protein (ECD, His & Fc Tag) (Cat: 10417-H03H), the EC50 is 5.2-15.5 ng/mL (Routinely tested) .

The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE. The molecular weight of this protein is around 50.3 kDa verified by SEC-MALS (Routinely tested) .