IL4 Protein (Native, Human)
Product Specifications
Abbreviation
IL4
Synonyms
BCGF-1, BCGF1, BSF-1, BSF1, IL-4, MGC79402
Host
E. coli
Tag
Native
Endotoxin
< 5 EU per mg of the protein.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Measured in a cell proliferation assay using TF‐1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of recombinant human IL-4 is >10,000 IU/μg which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656) . 2.Dendritic Cells (DCs) generation and maturation. CD14+ monocytes were differentiated into immature DCs by GM-CSF (Cat#GMP-10015-HNAH) and IL4 (Cat#GMP-11846-HNAE) for 6 days. DCs were matured by TNF (Cat#GMP-10602-HNAE) for 2 days. (Routinely tested) 3.Dendritic Cells (DCs) generation. CD14+ monocytes were differentiated into immature DCs by GM-CSF (Cat#GMP-10015-HNAH) and IL4 (Cat#GMP-11846-HNAE) for 6 days. (Routinely tested)
Function
His25-Ser153
Molecular Weight
The recombinant human IL4 consists of 130 amino acids and migrates with an apparent molecular mass of 15.1 kDa as predicted.
Species
Human
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
