IL4 Protein (Native, Human)

Product Specifications

Abbreviation

IL4

Synonyms

BCGF-1, BCGF1, BSF-1, BSF1, IL-4, MGC79402

Host

E. coli

Tag

Native

Endotoxin

< 5 EU per mg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.25 ng/mL. 2.Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with IL4 (Cat# 11846-HNAE) (100 ng/mL, 15min; +), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb (Cat# 111168-R0044) at 1:10000 dilution (upper) or Anti-STAT6 Antibody, Rabbit PAb (Cat# 201061-T36) at 1:1000 dilution (lower) (Routinely tested) . 3.Loaded Recombinant Human IL-4Rα & IL-13Rα1 Heterodimer Protein, hFc Tag (Cat. No. CT117-H02H) on proA Biosensor, can bind Recombinant Human IL-4 Protein (Cat. No. 11846-HNAE) with an affinity constant of 0.571 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Immobilized Recombinant Human IL4 / Interleukin-4 Protein (Cat: 11846-HNAE) at 5 μg/mL (100 μL/well) can bind Recombinant Human IL-4R / CD124 Protein (Fc Tag) (Cat: 10402-H02H), the EC50 is 17.3-51.9 ng/mL (QC tested) .