What is the catalog number for IL4 Protein (Native, Human)?

The catalog number for IL4 Protein (Native, Human) is P520309.

What sizes are available for IL4 Protein (Native, Human)?

IL4 Protein (Native, Human) is available in the following size(s): 100 µg.

Is IL4 Protein (Native, Human) in stock?

IL4 Protein (Native, Human) is currently made to order / not held in immediate stock. Contact us for lead time.

IL4 Protein (Native, Human)

Product Specifications

Abbreviation

IL4

Synonyms

BCGF-1, BCGF1, BSF-1, BSF1, IL-4, MGC79402

Host

E. coli

Tag

Native

Endotoxin

< 5 EU per mg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.25 ng/mL. 2.Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with IL4 (Cat# 11846-HNAE) (100 ng/mL, 15min; +), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb (Cat# 111168-R0044) at 1:10000 dilution (upper) or Anti-STAT6 Antibody, Rabbit PAb (Cat# 201061-T36) at 1:1000 dilution (lower) (Routinely tested) . 3.Loaded Recombinant Human IL-4Rα & IL-13Rα1 Heterodimer Protein, hFc Tag (Cat. No. CT117-H02H) on proA Biosensor, can bind Recombinant Human IL-4 Protein (Cat. No. 11846-HNAE) with an affinity constant of 0.571 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Immobilized Recombinant Human IL4 / Interleukin-4 Protein (Cat: 11846-HNAE) at 5 μg/mL (100 μL/well) can bind Recombinant Human IL-4R / CD124 Protein (Fc Tag) (Cat: 10402-H02H), the EC50 is 17.3-51.9 ng/mL (QC tested) .