FCGR3B Protein (C-His, Human)

1. Loaded Recombinant Human CD16B / FCGR3B Protein (NA1 allotype, His Tag, ) (Cat: 11046-H08H1) on His1K Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.99 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2. Loaded anti-CD20 (Research grade Rituximab Biosimilar) on ProA Biosensor, can bind Recombinant Human CD16B / FCGR3B Protein (NA1 allotype, His Tag, ) (Cat: 11046-H08H1) with an affinity constant of 0.16 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3. Recombinant Human CD16B / FCGR3B Protein (NA1 allotype, His Tag, ) (Cat: 11046-H08H1) captured on NTA chip, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.2 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4. Recombinant Human CD16B / FCGR3B Protein (NA1 allotype, His Tag, ) (Cat: 11046-H08H1) captured on CM5 Chip via Anti-his antibody chip, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 3.017 μM as determined in an SPR assay (Biacore T200) (QC tested) . 5. Loaded Recombinant Human CD16B / FCGR3B Protein (NA1 allotype, His Tag, ) (Cat: 11046-H08H1) on HIS1K Biosensor, can bind anti-CD20 (Research grade Rituximab Biosimilar) with an affinity constant of 10.5 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human FCGR3B consists of 195 amino acids and predicts a molecular mass of 22.4 kDa. As a result of glycosylation, it migrates as an approximately 35-45 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 27-39 kDa verified by SEC-MALS (Routinely tested) .