FCGR3A Protein (C-His, Human)

1. Loaded Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) on His1K Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.83 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2. Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) captured on CM5 Chip via Anti-his antibody chip, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.31 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3. anti-VEGFA (Research grade Bevacizumab Biosimilar) captured on ProA chip, can bind Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) with an affinity constant of 0.78 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4. Loaded Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) on His1K Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.81 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5. Loaded anti-CD20 (Research grade Rituximab Biosimilar) on ProA Biosensor, can bind Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) with an affinity constant of 0.622 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6. Loaded Recombinant Human CD16a / FCGR3A Protein (V176, His Tag) (Cat: 10389-H08H1) on HIS1K Biosensor, can bind anti-CD20 (Research grade Rituximab Biosimilar) with an affinity constant of 0.447 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Loaded Anti-CD20 (Research Grade Rituximab Biosimilar) (Cat. No. 68055-H005) on proL Biosensor, can bind Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein , V176, His Tag (Cat. No. 10389-H08H1) with an affinity constant of 1.430 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 8.Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein (F176V), His Tag (Cat. No. 10389-H08H1) captured on CM5 chip via Anti-his antibody, can bind Anti-HER2 (Research Grade Trastuzumab Biosimilar) with an affinity constant of 0.158 μM as determined in an SPR assay (Biacore T200) (QC tested) . 9.Anti-HER2 (Research Grade Trastuzumab Biosimilar) immobilized on CM5 chip, can bind Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein (F176V), His Tag (Cat. No. 10389-H08H1) with an affinity constant of 0.142 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human CD16a is approximately 44.2 KDa due to glycosylation.