FCGR3A Protein (C-AVI & His, Human)

Product Specifications

Abbreviation

FCGR3A

Synonyms

CD16, CD16-II, CD16A, FCG3, FcgammaRIIIa, FCGR3, FCGRIII, FcGRIIIA, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC

Bioactivity

1.Immobilized Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) (Cat. 10389-H27H1-B) at 10 μg/mL can bind recombinant human IgG1 (Fc) (Cat. 10702-HNAC) with a linear range of 0.31-5.0 μg/mL (Routinely tested) . 2. Loaded Recombinant Human CD16a/FCGR3A Protein (V176, His&AVI Tag), Biotinylated (Cat: 10389-H27H1-B) on SA Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 1.5 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 3.Loaded Biotinylated Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein (V176, His & AVI Tag) (Cat. No. 10389-H27H1-B) on SA Biosensor, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) (Cat. No. 68055-H005) with an affinity constant of 0.308 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Gln208

Molecular Weight

The secreted recombinant human CD16a consists of 227 amino acids and predicts a molecular mass of 25.6 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 KDa due to glycosylation.

Species

Human