FCGR2A Protein (C-His, Human)

Product Specifications

Abbreviation

FCGR2A

Synonyms

CD32, CD32A, CDw32, Fc-gamma-RIIa, FCG2, FcgammaRIIa, FcGR, FCGR2, FCGR2A1, IGFR2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

Bioactivity

1. Loaded anti-CD20 (Research grade Rituximab Biosimilar) on ProA Biosensor, can bind Recombinant Human CD32a / FCGR2A Protein (H167, His Tag) (Cat: 10374-H08H1) with an affinity constant of 1.7 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2. Loaded Recombinant Human CD32a / FCGR2A Protein (H167, His Tag) (Cat: 10374-H08H1) on His1K Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.55 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 3. Loaded Recombinant Human CD32a / FCGR2A Protein (H167, His Tag) (Cat: 10374-H08H1) on HIS1K Biosensor, can bind anti-CD20 (Research grade Rituximab Biosimilar) with an affinity constant of 0.871 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4. anti-VEGFA (Research grade Bevacizumab Biosimilar) captured on ProA chip, can bind Recombinant Human CD32a / FCGR2A Protein (H167, His Tag) (Cat: 10374-H08H1) with an affinity constant of 0.58 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Ile218

Molecular Weight

The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 KDa. It migrates as an approximately 30 KDa protein due to glycosylation by SDS-PAGE under reducing conditions.

Species

Human