FCGR2A Protein (C-His, Human)

1.Loaded anti-CD20 (Research grade Rituximab Biosimilar) on ProA Biosensor, can bind Recombinant Human CD32a / FCGR2A Protein (R167, His Tag) (Cat: 10374-H08H) with an affinity constant of 2.3 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Loaded Recombinant Human CD32a / FCGR2A Protein (R167, His Tag) (Cat: 10374-H08H) on His1K Biosensor, can bind anti-VEGFA (Research grade Bevacizumab Biosimilar) with an affinity constant of 0.65 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 3.anti-VEGFA (Research grade Bevacizumab Biosimilar) captured on ProA chip, can bind Recombinant Human CD32a / FCGR2A Protein (R167, His Tag) (Cat: 10374-H08H) with an affinity constant of 0.79 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.Recombinant Human CD32a / FCGR2A Protein (167 Arg, His Tag) (Cat: 10374-H08H) captured on CM5 chip via Anti-His antibody, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 5.262 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Loaded Recombinant Human IgG2-Fc Protein (257 Ser/Ala) (Cat. No. 13504-HNAH) on ProA Biosensor, can bind Recombinant Human CD32a / FCGR2A Protein (167 Arg, His Tag) (Cat. No. 10374-H08H) with an affinity constant of 4.05 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. The molecular weight of this protein is around 23-32 kDa verified by SEC-MALS (Routinely tested) .