CD3E Protein (C-His, Human)

Product Specifications

Abbreviation

CD3E

Synonyms

CD3-epsilon, CD3epsilon, IMD18, T3E, TCRE

Host

HEK 293

Tag

C-His

Endotoxin

< 0.05 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC. ≥ 85 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Anti-CD3 (Research Grade Clone SP34) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3e / CD3 epsilon Protein (His Tag) (Cat: 10977-H08H-UE), the EC50 is 1.5-4.5 ng/mL (Routinely tested) . 2.Loaded SP34 on ProA Biosensor, can bind Recombinant Human CD3e / CD3 epsilon Protein (His Tag) (Cat. No. 10977-H08H-UE) with an affinity constant of 4.25 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.SP34-derived anti-CD3E antibody, human IgG1 captured on Protein A chip, can bind Recombinant Human CD3e / CD3 epsilon Protein (His Tag) (Cat: 10977-H08H-UE) with an affinity constant of 0.736 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Asp126

Molecular Weight

The recombinant human CD3E consists of 115 amino acids after removal of the signal peptide and has a predicted molecular mass of 13.2 kDa. As a result of glycosylation, human CD3E migrates as an approximately 22.3 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 29.2 kDa verified by SEC-MALS (Routinely tested) .