CD3D & CD3E Protein (C-Flag & His, Human)

1.Flow cytometric analysis of anti-CD3 (OKT3 derived) CAR expression. 5e5 of anti-CD3 (OKT3 derived) CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human CD3D & CD3E Heterodimer Protein (Site-Specific PE Conjugation, Flag & His Tag, Cat. No. CT038-H2586H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left) . PE signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated CD3D & CD3E protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human CD3D & CD3E Heterodimer Protein (Site-Specific PE Conjugation, Flag & His Tag, Cat. No. CT038-H2586H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .