TNFRSF17 Protein (C-His, Human)

1.Immobilized Human BAFF (Cat:10056-H01H) at 2μg/mL (100 μl/well) can bind Human BCMA (Cat:10620-H08H), The EC50 of Human BCMA (Cat:10620-H08H) is 30-80 ng/mL (Routinely tested) . 2.Immobilized Mouse TNFSF13 (Cat:50965-M01H) at 2μg/mL (100 μl/well) can bind Human BCMA (Cat:10620-H08H), The EC50 of Human BCMA (Cat:10620-H08H) is 0.6-2.1 ng/mL (QC tested) . 3.293 cells were transduced with anti-BCMA-scFv and subjected to flow cytometric analysis using recombinant human BCMA (left, Cat. No. 10620-H08H, red) and a negative control protein (blue) . The cells were then stained with a PE-conjugated anti-His antibody. Non-transduced 293 cells were used as a control (right) (Routinely tested) . 4.Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag (Cat. No. 10056-H01H) on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 0.267 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Immobilized Anti-BCMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H), the EC50 is 3-10 ng/mL (Routinely tested) . 6.Anti-BCMA Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 22.3 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 7.Loaded Biotinylated Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag) (Cat. No. 10056-H56H1-B) on SA Biosensor, can bind Recombinant Human BCMA Protein (ECD, His Tag) (Cat. No. 10620-H08H) with an affinity constant of 0.853 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 8.Immobilized Recombinant Human APRIL/TNFSF13 Protein (Fc Tag) (Cat: 10610-H01H2) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H), the EC50 is 2.6 ng/mL (Routinely tested) . 9.Immobilized Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Antibody, the EC50 is 1.7 ng/mL (Routinely tested) .

The recombinant human TNFRSF17 consists of 65 amino acids and predicts a molecular mass of 7.3 kDa. As a result of glycosylation, It migrates as an approximately 16.9 and 12.4 KDa band in SDS-PAGE under reducing conditions.