Spike Protein (C-His, SARS-CoV-2)
Product Specifications
Abbreviation
Spike
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Bioactivity
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (40591-V08H), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (Cat: 40591-V08H) is 200-600 ng/mL. 2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.
Function
Val16-Arg685
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa.
Species
SARS-CoV-2
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