PCSK9 Protein (C-His, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized Human LDLR mFc (Cat:10231-H05H) at 2 μg/ml (100 μl/well) can bind Human PCSK9 (D374Y) His (Cat:29698-H08H1), the EC50 of PCSK9 (D374Y) His is 1-20 ng/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag (Cat. No. 10231-H05H) on ProA Biosensor, can bind Recombinant Human PCSK9 (D374Y) Protein, His Tag (Cat. No. 29698-H08H1) with an affinity constant of 2.57nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human LDLR/LDL Receptor Protein (Fc Tag) (Cat: 10231-H02H) at 2 μg/mL (100μL/well) can bind Recombinant Human PCSK9 / NARC1 (D374Y) Protein (His Tag) (Cat: 29698-H08H1), the EC50 is 8-25 ng/mL. Recombinant Human PCSK9 Protein (wild type, His Tag) (Cat: 29698-H08H2) is verified bind to Human LDLR/LDL Receptor Protein (Fc Tag) (Cat: 10231-H02H) wtih lower affinity (Routinely tested) .

The recombinant human PCSK9 consists of 673 amino acids and predicts a molecular mass of 72.5 kDa. As a result of glycosylation and proteolytic digestion, it migrates as doublet with apparent molecular mass of 19 kDa and 60 kDa corresponding to the pro domain and mature form respectively in SDS-PAGE under reducing conditions.