MSLN Protein (C-His, Human)

1.Flow cytometric analysis of anti-Mesothelin CAR expression. 5e5 of anti-Mesothelin CAR-293 cells were stained with 100 μL of 1:125 dilution of APC-conjugated human Mesothelin Protein (Site-specific APC-conjugated, His Tag, Cat. No. 13128-H86H1-SA) . Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated Mesothelin protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human Mesothelin Protein (Site-specific APC-conjugated, His Tag, Cat. No. 13128-H86H1-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .