GPC3 Protein (C-His, Human)

1.Flow cytometric analysis of anti-Glypican 3 CAR expression. 5e5 of anti-Glypican 3 CAR-293 cells were stained with 100 μL of 1 μg/mL of AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 647 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 647-conjugated Glypican 3 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .

The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.