GPC3 Protein (C-His, Human)

1.Flow cytometric analysis of anti-GPC3 CAR expression. 5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human GPC3 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 10088-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated GPC3 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human GPC3 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 10088-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .

The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.