EGFR Protein (C-His, Human)

1.Flow cytometric analysis of anti-EGFRvIII CAR expression. 5e5 of anti-EGFRvIII CAR-293 cells were stained with 100 μL of 1 μg/ml of AF 647-conjugated human EGFR Protein (Site-Specific AF 647 Conjugation, Isoform vIII, His Tag, Cat. No. 29662-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 647 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 647-conjugated EGFRvIII protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human EGFR Protein (Site-Specific AF 647 Conjugation, Isoform vIII, His Tag, Cat. No. 29662-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .