EGFR Protein (C-His, Human)

1.anti-EGFR (Research Grade Cetuximab biosimilar) immobilized on CM5 chip, can bind Recombinant Human EGFR Protein (Isoform vIII, His Tag) (Cat: 29662-H08H) with an affinity constant of 3.61 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 2.anti-EGFR (Research Grade Cetuximab biosimilar) captured on Protein A chip, can bind Recombinant Human EGFR Protein (Isoform vIII, His Tag) (Cat: 29662-H08H) with an affinity constant of 1.013 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Immobilized Recombinant Human EGFR Protein (Isoform vIII, His Tag) (Cat: 29662-H08H) at 1 μg/mL (100 μL/well) can bind anti-EGFR (Research Grade Cetuximab biosimilar), the EC50 is 0.6 ng/mL (Routinely tested) .

The recombinant human EGFR consists of 365 amino acids and predicts a molecular mass of 40.09 kDa. It migrates as an approximately 65.27 kDa band in SDS-PAGE under reducing conditions.