CD70 Protein (N-His, Human)

Product Specifications

Abbreviation

CD70

Synonyms

CD27-L, CD27L, CD27LG, LPFS3, TNFSF7, TNLG8A

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE.

Bioactivity

1.Flow cytometric analysis of anti-CD70 CAR expression. 5e5 of anti-CD70 CAR-293 cells were stained with 100 μL of 6μg/ml of AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 488 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 488-conjugated CD70 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .