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TNFSF15 Protein (N-His, Human)

Product Specifications

Abbreviation

TNFSF15

Synonyms

MGC129934, MGC129935, TL1, TL1A, TNLG1B, VEGI, VEGI192A

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-MALS.

Bioactivity

1.Immobilized Recombinant Human DCR3 / TNFRSF6B Protein (Fc Tag) (Cat: 10224-H02B) at 2 μg/mL (100 μL/well) can bind Recombinant Human TL1A/TNFSF15 Protein (His Tag) (Cat: 17049-H07H4), the EC50 is 3-10 ng/mL. 2.Loaded Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) on proA Biosensor, can bind Recombinant Human TL1A/TNFSF15 Protein, His Tag (Cat. No. 17049-H07H4) with an affinity constant of 0.689 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human TL1A/TNFSF15 Protein (His Tag) (Cat: 17049-H07H4), the EC50 is 70.2 ng/mL (Routinely tested) . 4.Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) captured on Protein A chip can bind Recombinant Human TL1A/TNFSF15 Protein (Trimer), His Tag (Cat. No. 17049-H07H4) with an affinity constant of 0.135 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Leu72-Leu251

Molecular Weight

The recombinant Human TNFSF15 comprises 199 amino acids and has a predicted molecular mass of 22.45 kDa. It migrates as approximately 30.9 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 94.9 kDa verified by SEC-MALS.

Species

Human

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