TNFSF15 Protein (N-His, Human)

1.Immobilized Recombinant Human DCR3 / TNFRSF6B Protein (Fc Tag) (Cat: 10224-H02B) at 2 μg/mL (100 μL/well) can bind Recombinant Human TL1A / TNFSF15 Protein (His Tag) (Cat: 17049-H07H2), the EC50 is 8-25 ng/mL. 2.Immobilized Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human TL1A / TNFSF15 Protein (His Tag) (Cat: 17049-H07H2), the EC50 is 20-110 ng/mL (Routinely tested) . 3.Measured by its ability to induce apoptosis of TF1 human erythroleukemic cells. The ED50 for this effect is typically 4-40ng/ml. 4.Recombinant Human DcR3/TNFRSF6B Protein , hFc Tag (Cat. No. 10224-H02B) captured on Protein A chip can bind Recombinant Human TL1A/TNFSF15 Protein (Trimer), His Tag (Cat. No. 17049-H07H2) with an affinity constant of 27.64 pM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) captured on Protein A chip can bind Recombinant Human TL1A/TNFSF15 Protein (Trimer), His Tag (Cat. No. 17049-H07H2) with an affinity constant of 0.117 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant Human TNFSF15 has a calculated molecular mass of 64.63 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 66.9 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 74.2 kDa verified by SEC-MALS.