Spike Protein (C-His-AVI, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 ((LAL242-244) deletion, L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G) Protein (His & AVI Tag), Biotinylated (Cat: 40591-V49H5-B), the EC50 of SARS-CoV-2 Spike S1 ((LAL242-244) deletion, L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G) Protein (His & AVI Tag), Biotinylated (Cat: 40591-V49H5-B) is 60-360 ng/mL.

Function

Met1-Arg685

Molecular Weight

The recombinant SARS-CoV-2 Spike S1 consists of 693 amino acids and predicts a molecular mass of 77.84 kDa. As a result of glycosylation, it migrates as an approximately 120.35 kDa band in SDS-PAGE under reducing conditions.