ROR1 Protein (C-His, Human)

1.Immobilized Anti-ROR1 (Research Grade Zilovertamab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human ROR1 Protein (ECD, His Tag) (Cat: 13968-H08H), the EC50 is 3.0-9.0 ng/mL (QC tested) . 2.Loaded Anti-ROR1 (Research Grade Zilovertamab Biosimilar) can bind Recombinant Human ROR1 Protein, His Tag (Cat. No. 13968-H08H) with an affinity constant of 19.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human ROR1 Protein (ECD, His Tag) (Cat: 13968-H08H) at 1 μg/mL (100 μL/well) can bind Anti-ROR1 (Research Grade Zilovertamab Biosimilar), the EC50 is 0.8 ng/mL (Routinely tested) . 4.Anti-ROR1 (Research Grade Zilovertamab Biosimilar) captured on Protein A chip, can bind Recombinant Human ROR1 Protein (ECD, His Tag) (Cat: 13968-H08H) with an affinity constant of 88.420 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human ROR1 consists of 385 amino acids and predicts a molecular mass of 43.4 kDa. As a result of glycosylation, it migrates as an approximately 55-66 KDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 56.3 kDa verified by SEC-MALS (Routinely tested) .