PDCD1 Protein (C-His, Human)

Product Specifications

Abbreviation

PDCD1

Synonyms

CD279, hPD-1, hPD-l, hSLE1, PD-1, PD1, SLEB2

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat: 10377-H08H-UE) at 2 μg/mL (100 μL/well) can bind Nivolumab-Derived Anti-PD1 (MDX) Antibody, human IgG4, the EC50 is 4.3 ng/mL. 2.Loaded Anti-PD1 (Research Grade Pembrolizumab Biosimilar) on ProA Biosensor, can bind Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat. No. 10377-H08H-UE) with an affinity constant of 13.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Gln167

Molecular Weight

The recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 30.6 kDa verified by SEC-MALS (Routinely tested) .