PDCD1 Protein (C-His, Human)

1.Immobilized human PD-1 (Cat:10377-H08H) at 2 μg/ml (100 μl/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10084-H02H), the EC50 of human B7-H1 is 150-600 ng/mL (QC tested) . 2.Immobilized human PD-1 (Cat:10377-H08H) at 2 μg/mL (100 μL/well) can bind Anti-PD1 (Research grade Nivolumab biosimilar), the EC50 is 8-60 ng/mL (Routinely tested) . 3.Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Recombinant Human PD1 Protein, His Tag (Cat. NO. 10377-H08H) with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind PD1 (Cat.No.10377-H08H) with an affinity constant of 10.03nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 5.Loaded Recombinant Human PD-L2/B7-DC Protein, hFc Tag (Cat. No. 10292-H02H) on proA Biosensor, can bind Recombinant Human PD-1 Protein, His Tag (Cat. No. 10377-H08H) with an affinity constant of 0.435 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Recombinant Human PD-1 Protein, His Tag (Cat. No. 10377-H08H) on HIS1K Biosensor, can bind Recombinant Human PD-L2/B7-DC Protein, hFc Tag (Cat. No. 10292-H02H) with an affinity constant of 19.400 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Immobilized Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat: 10377-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L2 / PDCD1LG2 Protein (ECD, mFc Tag) (Cat: 10292-H38H), the EC50 is 87.7 ng/mL (Routinely tested) . 8.Loaded Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat. No. 10377-H08H) on His1K Biosensor, can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat. No. 10084-H02H) with an affinity constant of 69.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 9.Loaded Recombinant Human PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag) (Cat. No. 10084-H05H) on AMC Biosensor, can bind Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat. No. 10377-H08H) with an affinity constant of 1.57 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions.