FOLH1 Protein (N-His, Human)

1.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >400 pmol/min/μg, as measured under the described conditions. 2.Loaded Monoclonal anti-human PSMA Antibody, human IgG4 on ProA Biosensor, can bind Recombinant Human PSMA/FOLH1 Protein, His Tag (Cat. No. 15877-H07H) with an affinity constant of 17.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized anti-PSMA at 2 μg/mL (100 μL/well) can bind Recombinant Human PSMA / FOLH1 Protein (ECD, His Tag) (Cat: 15877-H07H), the EC50 is 50-160 ng/mL.

The recombinant human FOLH1 consists 726 amino acids and predicts a molecular mass of 81.86 kDa. As a result of glycosylation, it migrates as an approximately 98.67 KDa band in SDS-PAGE under reducing conditions.