ERBB2 Protein (C-His, Human)

Product Specifications

Abbreviation

ERBB2

Synonyms

C-ERB-2, c-ERB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, MLN19, NEU, NGL, p185 (erbB2), TKR1, VSCN2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Bioactivity

1.Flow cytometric analysis of anti-ERBB2 CAR expression. 5e5 of anti-ERBB2 CAR-293 cells were stained with 100 μL of 2 μg/ml of AF 647-conjugated human ERBB2 Protein (Site-specific AF 647-conjugated, ECD, His Tag, Cat. No. 10004-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 647 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 647-conjugated ERBB2 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human ERBB2 Protein (Site-specific AF 647-conjugated, ECD, His Tag, Cat. No. 10004-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .