ERBB2 Protein (C-His, Human)
Product Specifications
Abbreviation
ERBB2
Synonyms
C-ERB-2, c-ERB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, MLN19, NEU, NGL, p185 (erbB2), TKR1, VSCN2
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Anti-Erbb2 Antibody (Trastuzumab), Human IgG1 at 2μg/mL (100μL/well) can bind Recombinant Human HER2 / ErbB2 Protein (ECD, His Tag), Biotinylated (Cat: 10004-H08H-B), the EC50 is 1-3 ng/mL. 2.293 cells were transduced with anti-ERBB2-scFv and subjected to flow cytometric analysis using biotinylated recombinant human ERBB2 (left, Cat. No. 10004-H08H-B, red) and a negative control protein (blue) . The cells were then stained with a FITC-conjugated Streptavidin. Non-transduced 293 cells were used as a control (right) .
Function
Met1-Thr652
Molecular Weight
The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, human ErbB2 migrates as an approximately 99 kDa protein in SDS-PAGE under reducing conditions.
Species
Human
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