ERBB2 Protein (C-His, Human)

1.Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag) (Cat:10004-H08H) at 2 μg/ml (100 μl/well) can bind Anti-HER2 (Research Grade Trastuzumab biosimilar), the EC50 is 10-60 ng/mL (Routinely tested) . 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody (Routinely tested) . 3.Captured anti-Her2 IgG1 antibody on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.270 nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 4.Captured Anti-HER2 (Research Grade Trastuzumab biosimilar) on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.727 nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 5.Loaded Anti-HER2 (Research Grade Pertuzumab biosimilar) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.382 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Anti-HER2 (Research Grade Trastuzumab biosimilar) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.547 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Immobilized Recombinant Human HER2 / ErbB2 Protein (His Tag) (Cat: 10004-H08H) at 1 μg/mL (100 μL/well) can bind Anti-HER2 (Research Grade Pertuzu*mab Biosimilar), the EC50 is 7.5 ng/mL (Routinely tested) .

The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 87.5 kDa verified by SEC-MALS (Routinely tested) .