EGFR Protein (C-His, Human)

1.Flow cytometric analysis of anti-EGFR CAR expression. 5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of APC-conjugated human EGFR Protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10001-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated EGFR protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human EGFR Protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10001-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .