EGFR Protein (C-His, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc (Cat: 10605-H01H) at 2 μg/ml (100 μl/well) can bind Human EGFR His (Cat: 10001-H08H), the EC50 of Human EGFR His is 100-500 ng/mL. 2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein (Cat. 10001-H08H) with an affinity constant of 1.07 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag (Cat. No. 10001-H08H) with an affinity constant of 5.81nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) .

The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.