CD4 Protein (C-His, Human)

1.Flow cytometric analysis of anti-CD4 CAR expression. 5e5 of anti-CD4 CAR-293 cells were stained with 100 μL of 5 μg/mL of AF 647-conjugated human CD4 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10400-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 647 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 647-conjugated CD4 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human CD4 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10400-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 3.1E5 of mouse Anti-CD4 antibody-conjugated beads (6-8 μm) were stained with a series of concentrations of AF 647-conjugated human CD4 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10400-H86H-SG) . Binding activity was quantified based on AF 647 fluorescence. The recommended working concentration is 2 μg/mL (end-user titration is recommended for optimal performance) .