CD4 Protein (C-His, Human)

1.Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to CD4-coated plates (0.8 μg/mL, 100 μL/well), approximately >40% will adhere specifically (Routinely tested) . 2.Immobilized Anti-CD4 (Research grade Ibalizumab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD4 / LEU3 Protein (His Tag) (Cat: 10400-H08H), the EC50 is 0.6-2.5 ng/mL (QC tested) . 3.Immobilized Anti-CD4 (Research Grade Ibalizu*mab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD4 / LEU3 Protein (His Tag) (Cat: 10400-H08H), the EC50 is 3.2 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human CD4 / LEU3 Protein (His Tag) (Cat: 10400-H08H) at 1 μg/mL (100 μL/well) can bind Anti-CD4 (Research Grade Ibalizu*mab Biosimilar), the EC50 is 11.0 ng/mL (Routinely tested) .

The recombinant human CD4 consists of 376 amino acids and has a predicted molecular mass of 42.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD4 is approximately 46 kDa due to glycosylation.