CD38 Protein (C-His, Human)

1.Flow cytometric analysis of anti-CD38 CAR expression. 5e5 of anti-CD38 CAR-293 cells were stained with 100 μL of 1:125 dilution of APC-conjugated human CD38 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10818-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated CD38 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD38 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10818-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 3.The site-specific APC conjugation specifically at the C-terminus (10818-H86H-SA) had better performance when binding to CAR that targeting epitopes distantal from the C-terminus (CAR02-293), and showed reduced binding affinity for proximal C-terminal epitopes (CAR01-293) .