CD276 Protein (C-His, Human)

1.Immobilized Enoblituzumab-Derived nti-Human B7H3 antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-H3 / CD276 Protein (ECD, His Tag) (Cat: 11188-H08H-UE), the EC50 is 6.7 ng/mL. 2.Loaded Anti-B7-H3 (Research Grade Enoblituzumab Biosimilar) on ProA Biosensor, can bind Recombinant Human B7-H3 / CD276 Protein (ECD, His Tag) (Cat. No. 11188-H08H-UE) with an affinity constant of 2.30 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD276 consists of 444 amino acids and predictes a molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CD276 is approximately 68 kDa due to glycosylation. The molecular weight of this protein is around 60.4 kDa verified by SEC-MALS (Routinely tested) .