CD276 Protein (C-His, Human)

Product Specifications

Abbreviation

CD276

Synonyms

4Ig-B7-H3, B7-H3, B7H3, B7RP-2, PSEC0249, UNQ309/PRO352

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Anti-Human B7H3 antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-H3 / CD276 Protein (ECD, His Tag) (Cat:11188-H08H), The EC50 is 2.5-7.5 ng/mL. 2.Loaded Enoblituzumab on proA Biosensor, can bind Recombinant Human B7-H3 Protein, His Tag (Cat. No. 11188-H08H) with an affinity constant of 1.06 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Thr461

Molecular Weight

The recombinant human CD276 consists of 444 amino acids and predictes a molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CD276 is approximately 68 kDa due to glycosylation. The molecular weight of this protein is around 61 kDa verified by SEC-MALS (Routinely tested) .