Spike Protein (C-human IgG1-Fc, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Immobilized human ACE2 protein (His tag) (10108-H08H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (R346K, E484K, N501Y) Protein (Fc Tag) (Cat: 40592-V02H6), the EC50 is 0.6-6.8 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 461 amino acids and predicts a molecular mass of 51.84 kDa. As a result of glycosylation, it migrates as an approximately 56.07 kDa band in SDS-PAGE under reducing conditions.