Spike Protein (C-human IgG1-Fc, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

Immobilized human ACE2 Protein, Human, Recombinant (His Tag) (Cat:10108-H08H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R, T478K) Protein (Fc Tag) (Cat:40592-V02H3), the EC50 of SARS-CoV-2 Spike RBD (L452R, T478K) Protein (Fc Tag) (Cat:40592-V02H3) is 1.0-8.0 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 461 amino acids and predicts a molecular mass of 51.89 kDa. As a result of glycosylation, it migrates as an approximately 58.07 kDa band in SDS-PAGE under reducing conditions.