MET Protein (C-His, Human)

Product Specifications

Abbreviation

MET

Synonyms

AUTS9, c-Met, DA11, DFNB97, HGFR, RCCP2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant Human HGF / Hepatocyte Growth Factor Protein (Cat: 10463-HNAS) at 2μg/mL (100μL/well) can bind Recombinant Human c-MET / HGFR Protein (His Tag) (Cat: 10692-H08H), the EC50 is 16-48 ng/mL.

Function

Met1-Thr932

Molecular Weight

The mature form of recombinant human c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 919 amino acids and has a predicted molecular mass of 103 (α=33 +β=70) kDa. As a result of glycosylation, rh c-MET heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 85 kDa respectively in SDS-PAGE under reducing conditions.