EpCAM Protein (C-His, Human)

Product Specifications

Abbreviation

EpCAM

Synonyms

17-1A,323/A3, Ber-Ep4, BerEp4, CD326, CO-17A, DIAR5, EGP-2, EGP314, EGP34, EGP40, Ep-CAM, EPCAM, ESA, GA733-2, HEA125, HNPCC8, KS1/4, KSA, Ly74, LYNCH8, M1S2, M4S1, MH99, MIC18, MK-1, MOC-31, MOC31, TACST-1, TACSTD1, TROP1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Bioactivity

1.Flow cytometric analysis of anti-EpCAM CAR expression. 5e5 of anti-EpCAM CAR-293 cells were stained with 100 μL of 2 μg/ml of AF 647-conjugated human EpCAM Protein (Site-specific AF 647-conjugated, ECD, His Tag, Cat. No. 10694-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left) . AF 647 signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of AF 647-conjugated EpCAM protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human EpCAM Protein (Site-specific AF 647-conjugated, ECD, His Tag, Cat. No. 10694-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .