CEACAM5 Protein (C-His, Human)

1.Loaded Anti-Human CEACAM5 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human CEACAM5 Protein, His Tag (Cat. No. 11077-H08H) with an affinity constant of 3.72 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Immobilized Anti-CEA Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human CEACAM5 / CD66e Protein (His Tag) (Cat:11077-H08H), The EC50 is 5.5-16 ng/mL. 3.Anti-CEA Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human CEACAM-5/CD66e Protein, His Tag (Cat. No. 11077-H08H) with an affinity constant of 3.22 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The secreted recombinant human CEACAM5 consists of 662 amino acids and has a predicted molecular mass of 72.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CEACAM5 is approximately 128 and 154.7 kDa due to different glycosylation.