CD33 Protein (C-His, Human)

1.Flow cytometric analysis of anti-CD33 CAR expression. 5e5 of anti-CD33 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human CD33 Protein (APC site-specific conjugation, ECD, His Tag, Cat. No. 12238-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated CD33 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD33 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 12238-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .