CD33 Protein (C-His, Human)

Product Specifications

Abbreviation

CD33

Synonyms

CD33rSiglec, FLJ00391, p67, SIGLEC-3, SIGLEC3

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-CD33 Antibody (Lintuzumab), Human IgG1 at 2 μg/ml (100 μl/well) can bind Recombinant Human CD33 / Siglec-3 Protein (ECD, His Tag) (Cat: 12238-H08H), the EC50 is 1.5-6 ng/mL. 2.Loaded anti-CD33 antibody on ProA Biosensor, can bind Recombinant Human Siglec-3/CD33 Protein, His Tag (Cat. No. 12238-H08H) with an affinity constant of 0.379 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-His259

Molecular Weight

The recombinant human CD33 comprises 253 amino acids and has a predicted molecular mass of 28.2 kDa. The apparent molecular mass of the protein is approximately 40-46 kDa in SDS-PAGE under reducing conditions due to glycosylation.