ACE2 Protein (C-His, Human)

Product Specifications

Abbreviation

ACE2

Synonyms

ACEH, UNQ868/PRO1885

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Loaded Recombinant SARS-CoV-2 Spike RBD-mFc Recombinant Protein (HPLC-verified) (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 Protein (His Tag) (HPLC-verified) (Cat. No. 10108-H08H-UE) with an affinity constant of 3.23 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Recombinant SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein (Cat: 40591-V02H) captured on Protein A chip, can bind Recombinant Human ACE2 Protein (His Tag) (HPLC-verified) (Cat: 10108-H08H-UE) with an affinity constant of 1.386 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Ser740

Molecular Weight

The recombinant human ACE2 consists of 734 amino acids and predicts a molecular mass of 85.1 kDa. As a result of glycosylation, it migrates as an approximately 100-120 kDa band in SDS-PAGE under reducing conditions.

Species

Human