ACE2 Protein (C-His, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat: 40592-V05H) at 2 μg/mL (100 μL/well) can bind human ACE2 protein (His tag), the EC50 of ACE2 is 10-30 ng/mL. 2. Measured by its ability to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp) OH, R&D Systems, Catalog # ES007. The specific activity is >1000 pmols/min/μg. 3.Loaded Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 Protein, His Tag (Cat. No. 10108-H08H) with an affinity constant of 2.46 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 Protein, Fc Tag (Cat. No. 40591-V02H) captured on Protein A chip can bind Recombinant Human ACE2 Protein, His Tag (Cat. No. 10108-H08H) with an affinity constant of 1.50 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .