Spike Protein (C-His, SARS-CoV-2)
Product Specifications
Abbreviation
Spike
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Bioactivity
1. Immobilized human ACE2 protein (mFc tag) (Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 (Omicron) S1+S2 trimer Protein (ECD, His Tag) (Cat:40589-V08H26), the EC50 is 4-15 ng/mL. 2. Load human ACE2, mFc tag (Cat:10108-H05H) on AMC biosensor, can bind SARS-CoV-2 B.1.1.529 (Omicron) S1+S2 trimer Protein (ECD, His Tag) (Cat:40589-V08H26) with an affinity constant of 1.52 nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested) .
Molecular Weight
The recombinant SARS-CoV-2 Spike S1+S2 consists of 1228 amino acids and predicts a molecular mass of 136.67 kDa.
Species
SARS-CoV-2
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