CD274 Protein (C-His, Human)

1.Immobilized Anti-PDL1 Antibody (Research Grade Atezolizumab Biosimilar) at 2 μg/mL (100μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat: 10084-H08H-UE) . The protein exhibited stable performance after 21 days of storage at 37°C, as measured on Day 0, Day 7, Day 14 and Day 21. 2.Immobilized Anti-PDL1 Antibody (Research Grade Atezolizu*mab Biosimilar) at 2 μg/mL (100μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat: 10084-H08H-UE), the EC50 is 0.8-2.5 ng/mL (Routinely tested) . 3.Loaded Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat. No. 10377-H02H) on ProA Biosensor, can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat. No. 10084-H08H-UE) with an affinity constant of 0.101 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the human B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 31.9 kDa verified by SEC-MALS (Routinely tested) .