CD274 Protein (C-His, Human)

Product Specifications

Abbreviation

CD274

Synonyms

B7-H, B7-H1, B7H1, hPD-L1, PD-L1, PDCD1L1, PDCD1LG1, PDL1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-PDL1 (GEN) -IgG1 Antibody (Atezolizumab) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat:10084-H08H), the EC50 is 0.7-2.1 ng/mL (QC tested) . 2. Loaded Recombinant Human PD-1 Protein, hFc Tag (Cat. No. 10377-H02H) on ProA Biosensor, can bind Recombinant Human PD-L1/B7-H1 Protein, his Tag (Cat. No. 10084-H08H) with an affinity constant of 1.4 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Captured Anti-PD-L1 Mab (Human IgG1) on proA Chip can bind PD-L1 (Cat.No.10084-H08H) with an affinity constant of 1.381nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 4.Recombinant Human PD-1 Protein, hFc Tag (Cat. No. 10377-H02H) captured on Protein A chip, can bind Recombinant Human PD-L1/B7-H1 Protein, His Tag (Cat. No. 10084-H08H) with an affinity constant of 1.620 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Immobilized Recombinant Human PD-L1/B7-H1 Protein (ECD, His Tag), HPLC-verified (Cat: 10084-H08H) at 1 μg/mL (100 μL/well) can bind Atezolizumab derived Anti-PDL1 Antibody , Human IgG1, the EC50 is 3-9 ng/mL (Routinely tested) .

Function

Met1-Thr239

Molecular Weight

The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the human B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.