CD19 Protein (C-His, Human)

1.Flow cytometric analysis of anti-CD19 CAR expression. 5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:125 dilution of PE-conjugated human CD19 Protein (Site-Specific PE-Conjugated, ECD, His Tag, Cat. No. 11880-H08W1-SP) and negative control protein. Non-transduced 293 cells were used as a control (left) . PE signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated CD19 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human CD19 Protein (Site-Specific PE-Conjugated, ECD, His Tag, Cat. No. 11880-H08W1-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 3.Flow cytometric analysis of anti-CD19 CAR expression. HEK293 cells were lentivirally transduced with anti-CD19 CAR. Non-transduced HEK293 cells were used as a control (shown on the left) . 2 μL (1 test) of site-specifically PE-conjugated human CD19 protein from two vendors (competitor A and SINO) was used. The results demonstrated that SINO’s 11880-H08W1-SP has a much higher fluorescence intensity than that of the competitor (Routinely tested) . 4.Flow cytometric analysis using PE conjugated CD19 protein. 5e5 of anti-CD19 CAR-293 cells were stained with PE-conjugated human CD19 protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SP) . Non-transduced 293 cells were used as a control (Black Histogram) . The protein exhibited stable performance after multiple freeze-thaw cycles (5, 10 cycles) . 5.Flow cytometric analysis using different lots of PE conjugated CD19 protein. 5e5 of anti-CD19 CAR-293 cells were stained with 3 lots of PE-conjugated human CD19 protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SP) at the same concentration. Non-transduced 293 cells were used as a control (Black Histogram) . The results show high lot-to-lot consistency.